白三叶CHS基因的过表达提高烟草类黄酮的含量
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  • 作者:吴榕 ; 田再民 ; 郑国华 ; 吴华圃 ; 吴艳玲 ; 牛艳芳
  • 关键词:白三叶 ; 查尔酮合成酶 ; 烟草遗传转化 ; 类黄酮
  • 中文刊名:草业科学
  • 英文刊名:Pratacultural Science
  • 机构:内蒙古师范大学生命科学与技术学院;河北北方学院农林科技学院;内蒙古呼和浩特蔬菜推广站;呼和浩特市赛罕区农牧业局;
  • 出版日期:2020-02-15
  • 年:2020
  • 期:02
  • 基金:内蒙古自然科学基金(2017MS0365);; 国家自然科学基金(31660157);; 内蒙古自治区高等学校研究项目(NJZY17044)
  • 页:99-107
  • CN:62-1069/S
  • ISSN:1001-0629
  • 分类号:S54
摘要
为探索白三叶(Trifolium repens)查尔酮合成酶基因对类黄酮含量的影响,根据NCBI上提供的TrCHS基因(Genbank:2247906)序列,采用RT-PCR方法克隆获得白三叶查尔酮合成酶(chalcone synthase, CHS)基因全长c DNA片段,命名为TrCHS;成功构建含有TrCHS的植物表达载体,在烟草(Nicotiana)中进行遗传转化,经抗性筛选与分子检测,获得了转基因烟草株系,并对其类黄酮含量进行了测定。结果表明,与野生型相比,转基因烟草植株TrCHS表达量和类黄酮含量均显著提高(P <0.05),表明过表达TrCHS可提高烟草类黄酮的含量,这为后续解析TrCHS功能以及白三叶新品种选育提供了可靠的理论依据。
    
引文
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