棉铃虫几丁质合成酶1基因的时空表达分析及氟啶脲对其表达的影响
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  • 作者:台术蕾 ; 马龙 ; 张春妮
  • 关键词:棉铃虫 ; 几丁质合成酶1基因 ; 时空表达 ; 氟啶脲
  • 中文刊名:环境昆虫学报
  • 英文刊名:Journal of Environmental Entomology
  • 机构:西北农林科技大学植物保护学院;
  • 出版日期:2020-01-05
  • 年:2020
  • 期:01
  • 基金:国家自然科学基金(31672037)
  • 页:140-149
  • CN:44-1640/Q
  • ISSN:1674-0858
  • 分类号:S435.622.3
摘要
本研究旨在明确棉铃虫Helicoverpa armigera(Hübner)几丁质合成酶1(HaCHS1)基因在不同发育时期和不同组织的表达及氟啶脲处理后对该基因表达的影响。利用RT-PCR和RACE技术克隆获得棉铃虫HaCHS1基因,HaCHS1基因全长为5 247 bp,其中开放阅读框4 698 bp,编码1 565个氨基酸,5′非翻译区为168 bp,3′非翻译区为381 bp。预测蛋白的相对分子质量为180.7 kDa,等电点为6.50,HaCHS1具有16个跨膜螺旋和6个N-糖基化位点。RT-qPCR检测结果表明,HaCHS1基因在预蛹期表达量最低,在蛹期第1天表达量最高。HaCHS1基因在头部的表达量最高,其次是表皮,而在中肠、气管、马氏管和脂肪体中的表达量均极低。亚致死浓度(60 mg/L)氟啶脲处理后,棉铃虫HaCHS1基因呈现出"抑制-激活-再抑制"的波动。本研究将为棉铃虫HaCHS1基因功能的研究奠定基础,为基于几丁质合成途径设计新药提供科学的理论依据。
    
引文
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