脂肪间充质干细胞移植对颞叶癫痫大鼠血清及海马组织SOD和MDA的影响
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  • 英文篇名:Effects of adipose-derived stem cells transplantation on SOD and MDA in serum and hippocampus in rats with temporal lobe epilepsy
  • 作者:王丽 ; 张宁 ; 王桂松 ; 赵迎春 ; 潘晓春
  • 英文作者:WANG Lili;ZHANG Ning;WANG Guisong;Department of Neurology,Songjiang District Central Hospital;
  • 关键词:颞叶癫痫 ; 脂肪间充质干细胞 ; 血清超氧化物岐化酶 ; 丙二醛 ; 海马
  • 英文关键词:Temporal lobe epilepsy;;Adipose-derived stem cells;;Superoxide dismutase;;Malondialdehyde;;Hippocampus
  • 中文刊名:江苏医药
  • 英文刊名:Jiangsu Medical Journal
  • 机构:上海松江区中心医院神经内科;上海交通大学附属第六人民医院南院神经外科;上海交通大学附属仁济医院神经外科;
  • 出版日期:2020-02-28
  • 年:2020
  • 期:02
  • 基金:上海市自然科学基金(04ZR14070)
  • 页:11-13
  • CN:32-1221/R
  • ISSN:0253-3685
  • 分类号:R742.1
摘要
目的探讨脂肪间充质干细胞(ADSCs)移植对颞叶癫痫大鼠血清及海马组织超氧化物岐化酶(SOD)活力和丙二醛(MDA)含量的影响。方法 40只SD大鼠分为移植组(n=15)、假移植组(n=15)和对照组(n=10)。通过立体定向坐标,用微量注射器将ADSCs注入大鼠左侧海马CA3区,检测各组大鼠血清及海马组织SOD活力和MDA含量。结果与对照组和移植组比较,假移植组大鼠的血清和海马组织的MDA含量升高,SOD活力下降(P<0.05),移植组与对照组大鼠血清和海马组织MDA含量和SOD活力无统计学差异(P>0.05)。结论 ADSCs移植治疗颞叶癫痫大鼠的机制可能与增加血清及海马组织中SOD活力,降低MDA含量,从而改善氧化应激反应有关。
    Objective To investigate the effects of adipose-derived mesenchymal stem cells(ADSCs)transplantation on superoxide dismutase(SOD)activity and malondialdehyde(MDA)content in serum and the hippocampus in rats with temporal lobe epilepsy.Methods Forty SD rats were divided into three groups of A(ADSCs transplantation,n=15),B(sham transplantation,n=15)and C(control,n=10).By stereospecific coordinates,ADSCs were injected into hippocampal CA3 region on the left side of the rats by a microsyringe.SOD activity and MDA content in serum and the hippocampus were detected in three groups.Results Compared with groups of C and A,MDA content in serum and the hippocampus in group B were higher,while SOD activity in serum and the hippocampus were lower significantly(P<0.05).MDA content and SOD activity in serum and the hippocampus were not significantly different between groups of A and C(P>0.05).Conclusion The mechanism for ADSCs transplantation treating temporal lobe epilepsy may be related to the increase of SOD activity and the decrease of MDA content in serum and the hippocampus,which reflects the improvement of oxidative stress.
引文
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