脂肪间充质干细胞转化神经元移植对颞叶癫疒间大鼠学习记忆的影响
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  • 英文篇名:Effects of adipose-derived stem cell-transformed neuron transplantation on learning and memory in temporal lobe epilepsy rats
  • 作者:王丽 ; 梅广海 ; 张宁 ; 王桂松 ; 赵迎春 ; 潘晓春 ; 陈红专 ; 朱亮
  • 英文作者:Wang Lili;Mei Guanghai;Zhang Ning;Wang Guisong;Zhao Yingchun;Pan Xiaochun;Chen Hongzhuan;Zhu Liang;Department of Neurology, Songjiang Branch of the First People's Hospital Affiliated to Shanghai Jiaotong University;Department of Neurosurgery, Huashan Hospital Affiliated to Shanghai Fudan University;Department of Neurosurgery, South Hospital of the Sixth People's Hospital Affiliated to Shanghai Jiaotong University;Department of Neurosurgery, Renji Hospital Affiliated to Shanghai Jiaotong University;Laboratory of Pharmacology, Basic Medical College, Shanghai Jiaotong University;
  • 关键词:癫疒间 ; 颞叶 ; 脂肪间充质干细胞 ; Morris水迷宫 ; 学习 ; 记忆 ; 大鼠
  • 英文关键词:epilepsy,temporal lobe;;adipose-derived stem cells;;Morris water maze;;learning;;memory;;rats
  • 中文刊名:中国微侵袭神经外科杂志
  • 英文刊名:Chinese Journal of Minimally Invasive Neurosurgery
  • 机构:上海交通大学附属第一人民医院松江分院神经内科;上海复旦大学附属华山医院神经外科;上海交通大学附属第六人民医院南院神经外科;上海交通大学附属仁济医院神经外科;上海交通大学基础医学院药理学教研室;
  • 出版日期:2020-02-20
  • 年:2020
  • 期:02
  • 页:36-40
  • CN:44-1459/R
  • ISSN:1009-122X
  • 分类号:R742.1
摘要
目的探讨移植脂肪间充质干细胞(adipose-derived stem cells,ADSC)转化神经元移植对颞叶癫疒间大鼠学习记忆的影响。方法 SD大鼠按实验要求,随机分成4组:移植组(n=15,颞叶癫疒间大鼠造模后在海马植入ADSC转化神经元),假移植组(n=15,颞叶癫疒间大鼠造模后在海马注射生理盐水),NS组(n=10,实验大鼠不造模,仅在海马注射生理盐水)和ADSC对照组(n=10,实验大鼠不造模,仅在海马植入ADSC转化神经元)。移植前、移植后2周、移植后2个月,分别进行Morris水迷宫实验,比较大鼠学习记忆能力的变化。结果移植后2周及2个月,与假移植组相比,移植组大鼠寻找平台平均潜伏期均缩短(P <0.05、P <0.01);而与NS组及ADSC对照组相比,无显著性差异(均P>0.05)。移植后2周及2个月,与NS组比较,移植组和ADSC对照组大鼠穿越Ⅱ象限时间,均无显著性差异(均P>0.05);而与移植组比较,假移植组大鼠穿越Ⅱ象限时间明显缩短(P <0.01)。结论 ADSC转化神经元移植能改善颞叶癫疒间大鼠的学习记忆能力,且无明显副作用,具体机制尚待进一步研究。
    Objective To investigate the effects oftransplantation ofadipose-derived stem cells(ADSCs) transformed neurons on the learning and memory of temporal lobe epilepsy rats. Methods The SD rats were randomly divided into 4 groups according to the experimental requirements: transplantation group(n = 15, ADSC-transformed neurons were implanted into the hippocampus after modeling of temporal lobe epilepsy rats), sham-transplantation group(n = 15, temporal lobe epilepsy rats after modeling, saline was injected into the hippocampus), NS group(n = 10, experimental rats were not modeled, saline was injected only in the hippocampus) and ADSC control group(n = 10, experimental rats were not modeled, only ADSC-transformed neurons were implanted into the hippocampus). Before transplantation, 2 weeks and 2 months after transplantation, Morris water maze tests were performed to compare the changes in learning and memory abilities of rats. Results After 2 weeks and 2 months of transplantation, compared with the sham-transplantation group, the average latency of finding platform in the transplantation group was shorter(P <0.05, P <0.01); compared with the NS group and the ADSC control group, there was no significant difference(both P> 0.05). After 2 weeks and 2 months of transplantation, compared with the NS group, there was no significant difference in the time across quadrantⅡ between the transplantation group and the ADSC control group(both P> 0.05); compared with the transplantation group, the time across quadrantⅡof the rats in the sham-transplantation group was significantly shorter(P <0.01). Conclusions The ADSC-transformed neuron transplantation can improve the learning and memory ability of temporal lobe epilepsy rats without significant side effects. The specific mechanism needs further study.
引文
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