湖南柑橘根结线虫种类鉴定及特异性PCR检测
摘要
根结线虫病是严重危害湖南柑橘生产的主要病害,快速准确地进行病原线虫鉴定,从而制定针对性的防治措施,对柑橘产业的稳定发展至关重要。运用形态学和分子生物学技术对湖南永州地区柑橘根结线虫进行病原种类鉴定,确定其为番禺根结线虫Meloidogyne panyuensis。通过引物设计与筛选,建立了番禺根结线虫特异性PCR检测技术。结果表明,该检测方法特异性好,灵敏度高,操作简单,能有效地从多种根结线虫中特异性检测出番禺根结线虫,为柑橘病原线虫的快速检测鉴定提供技术支撑。
Root-knot nematode is a dangerous disease imposing significant effects on citrus growth and yield in Hunan province. The root-knot nematode was collected from infested citrus rhizome in Yongzhou, Hunan province. Based on PCR amplification and morphological observation, the root-knot nematode was identified as Meloidogyne panyuensis. According to rDNA-ITS sequence alignment, a set of primers was designed to develop a species-specific molecular tool for the precise identification of M.panyuensis. The primer reliability, specificity and sensitivity tests showed that the specific PCR was highly sensitive, specific and simple, and could effectively detect M.panyuensis from various root-knot nematodes. This specific and rapid PCR detection technology may be a powerful tool for improving taxonomic identification of citrus pathogen nematodes.
引文
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