豌豆种传花叶病毒检测鉴定

详细信息    查看全文 | 推荐本文 | 收藏本文

• 英文篇名：Detection and identification of pea seed-borne mosaic virus
• 作者：王仲敏 ; 柴铭骏 ; 胡佳续 ; 张莹 ; 廖芳
• 英文作者：Wang Zhongmin;Chai Mingjun;Hu Jiaxu;Zhang Ying;Liao Fang;Animal,Plant,and Foodstuffs Inspection Center of Tianjin Custom District;
• 关键词：豌豆种传花叶病毒 ; 双抗体夹心酶联免疫吸附 ; 反转录PCR ; 序列分析
• 英文关键词：pea seed-borne mosaic virus;;DAS-ELISA;;RT-PCR;;sequence analysis
• 中文刊名：植物检疫
• 英文刊名：Plant Quarantine
• 机构：天津海关动植物与食品检测中心;
• 出版日期：2020-01-15
• 年：2020
• 期：01
• 基金：国家重点研发计划国家质量基础(NQI)课题(2017YFF0210305);; 原国家质检总局科技计划项目(2017IK215);; 科技部十二五科技支撑计划课题(2012BAK11B02)
• 页：24-26
• CN：11-1990/S
• ISSN：1005-2755
• 分类号：S432.41

摘要

豌豆种传花叶病毒(pea seed-borne mosaic virus,PSbMV)世界广泛分布,在国外已造成豌豆大量减产,国内有仅小范围低密度分布的报道。加拿大豌豆种子中挑拣皱缩、黑斑等症状的豌豆,先采用双抗体夹心酶联免疫吸附(DAS-ELISA)方法检出PSbMV阳性,再利用反转录PCR(RT-PCR)、序列比对分析和构建系统进化树对阳性样本进行验证鉴定,结果显示与PSbMV序列相似度为99.25%,综上确定从进境豌豆种子中检出豌豆种传花叶病毒。
    Pea seed-borne mosaic virus is widely distributed in the world causing a large reduction in pea production,and is reported with only small range and low density distribution in China. Samples were seleted from Canada pea seeds with signs of shrinkage and black spots. DAS-ELISA detection revealed that these samples reacted positively with the PSbMV antibody. For further confirmation PSbMV infection,RT-PCR,sequence alignment and phylogenetic analysis were used to verify and identify the ELISA-positive samples. The results showed that the virus from pea seeds shared 99.25% similarity with the PSbMV. It confirmed that imported pea seeds are infected with PSbMV.

引文

[1] Congdon B S,Coutts B A,Renton M,et al. Establishing alighting preferences and species transmission differences for Pea seed-borne mosaic virus aphid vectors. Virus Research,2017,241(15):145-155.
    [2] Congdon B S,Flematti G R,Jones R A C. Forecasting model for,Pea seed-borne mosaic virus,epidemics in field pea crops in a Mediterranean-type environment. Virus Research,2017,153(5):78-88.
    [3] Stevenson W R. Effect of Seed Size and Condition on Transmission of Pea seed-borne mosaic virus. Phytopathology,1970,60(7):1148-9.
    [4] Cerna H,Cˇern伥M,HabánováH,et al. Proteomics offers insight to the mechanism behind Pisum sativum L. response to Pea seed-borne mosaic virus(PSb MV). Journal of Proteomics,2017,153(5):78-88.
    [5]周雪平濮祖芹.侵染豌豆的两种病毒病的初步鉴定.南京农业大学学报,1990,(s1):64-67.
    [6]桂晋刚.大豆花叶病毒P基因分子变异的初步研究及豌豆种传花叶病毒在我国豌豆品系中的鉴定[D].北京:中国科学院遗传学研究所;中国科学院遗传与发育生物学研究所,1999.
    [7] Marie-Jeanne V,Ioos R,Peyre J,et al. Differentiation of Poaceae Potyviruses by Reverse Transcription‐Polymerase Chain Reaction and Restriction Analysis. Journal of Phytopathology,2008,148(3):141-151.
    [8]陆建英杨晓明.豌豆种传花叶病毒病研究综述.甘肃农业科技,2013(9):50-53.
    [9]胡丽云.豌豆高产优质栽培技术.中国农村小康科技,2007,(9):58.