14种柑橘皮中多甲氧基黄酮成分分析
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  • 英文篇名:Analysis of Polymethoxy?avones in Citrus Peels of Different Varieties
  • 作者:许引 ; 吕小健 ; 李明 ; 龙涛 ; 徐兰英 ; 李士明
  • 英文作者:XU Yin;Lü Xiaojian;LI Ming;LONG Tao;XU Lanying;LI Shiming;College of Chemical and Chemical Engineering, Wuhan Textile University;Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemical and Chemical Engineering,Huanggang Normal University;
  • 关键词:柑橘皮 ; 多甲氧基黄酮 ; 陈皮 ; 高效液相色谱
  • 英文关键词:citrus peels;;polymethoxy?avones;;Pericarpium Citri Reticulatae;;high performance liquid chromatography
  • 中文刊名:食品科学
  • 英文刊名:Food Science
  • 机构:武汉纺织大学化学与化工学院;黄冈师范学院化学化工学院催化材料制备及应用湖北省重点实验室;
  • 出版日期:2019-01-18 08:58
  • 年:2019
  • 期:08
  • 基金:国家自然科学基金面上项目(31571832)
  • 页:144-148
  • CN:11-2206/TS
  • ISSN:1002-6630
  • 分类号:R284;O657.72
摘要
建立柑橘皮中9种多甲氧基黄酮(polymethoxyflavonoids,PMFs)快速定量检测方法,比较不同品种来源柑橘皮中PMFs的含量差异,为寻找高含量PMFs高生物活性柑橘皮的药食两用素材提供参考。采用超声乙醇提取、高效液相色谱方法快速准确检测PMFs,并对14种柑橘皮、陈皮中9种特征物质PMFs含量进行比较。结果显示9种PMFs在对照品溶液质量浓度范围内具有良好的线性关系,且准确度和精确度良好。分析发现,不同品种来源柑橘皮中PMFs种类和含量差异很大。江西陈皮、重庆柑橘、广西皇帝柑、广西砂糖橘果皮中PMFs总含量很高,均在7 495.19 μg/g以上,其中广西沙糖橘果皮PMFs总含量高达10 412.18 μg/g。该方法简便,稳定性好,可为柑橘皮中PMFs生物活性研究的深入、柑橘皮产品开发和中药原料选材及其质量控制提供科学依据。
    This study aimed to develop a rapid method for the quanti?cation of nine polymethoxy?avones(PMFs) in the peels of fourteen citrus varieties. The citrus peel samples were compared for their PMFs contents in order to provide useful data for discovering PMFs-rich citrus peels with potent bioactivity for both medicinal and culinary purposes. Citrus peel extracts were prepared by ultrasonic-assisted extraction using ethanol as a solvent. A rapid and accurate high performance liquid chromatography(HPLC) method was used to measure the content of PMFs in citrus peels. The method showed good linearity repeatability and precision. The types and quantities of PMFs greatly varied with citrus variety. Pericarpium Citri Reticulatae from Citrus reticulata Kinokuni and the dried peels of Chongqing tangerine, Guangxi Emperor tangerine and Shatangju mandarin contained high contents of PMFs(more than 7 495.19 μg/g). Notably, total PMFs of Shatangju mandarin peel was as high as 10 412.18 μg/g. The HPLC method is simple and reliable and can provide a useful approach for study of PMFs in citrus peels.
引文
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